Examination of Chemical Compounds in a bacterium Micrococcus Using Methyl RedCompounds usedA. Compound name: Methyl Red (Methyl Red)Chemical Name: Dibromo-thymol-sulfonftaleino-hydroxy-difenilsulfonftalein
Methyl Red (Methyl Red) is an organic compound that has the chemical formula C15H15N3O2, these compounds are widely used for acid-base indicator. This indicator is red at pH 4.4 and yellow at pH above 6.2. transition produces the color orange. Methyl Red is made with diazotisasi of antranilat acid followed by reaction with dimethylaniline. Here's a picture of making the reaction of methyl red.
Source: en.wikipedia.org / wiki / Methyl_RedB. Structure of Chemical Compounds
The chemical structure of methyl redSources: http://belajarkimia.com/kamuskimia/wp-content/uploads/2010/07/metilmerah.png
C. Chemical properties of Methyl Red
A. Tangible dark red crystalline powder.2. Stable. Incompatible with strong oxidizing agents.
D. Physical properties of compoundsMolecular formula: C15H15N3O2Molar mass: 269.3 g / molDensity: 0.791 gram/cm3Melting point: 179-182 ⁰ CDensity: 0839 g / mL at 25 ⁰ C
Chapter II: Chemical Changes in the Examination of Methyl Red Using Micrococcus2. Chemical changes in the testIn this test, the bacteria Micrococcus and media used methyl red. The goal is to know the reaction that occurs between the bacteria Micrococcus methyl red in the media. Micrococcus bacteria are gram-positive bacteria are not motile catalase positive, negative oxidation, methyl red positive, positive optimum growth temperature at 30 ⁰ -37 ⁰ C and grows well in 1-7% NaCl. Cells form a ball, diameter 0.5 to 2.0 μm, in the form of a pair, four pairs, or groups are not fixed, do not form chains. Micrococcus will usually grow on simple media. If that were cultured in agar-agar or nutrient rich, such as blood for blood. The following composition of Micrococcus isolation media:Composition per liter:
In order for 15 gramsPeptone 5 g3 grams of yeast extract1.5 grams of beef extractGlucose 1 gram
Media images of isolation for Micrococcus
A. Characteristics of Chemical Changes
Micrococcus has the aerobic capacity to produce acid from glucose glycerol, aesculin hydrolysis, arginine dihydrolase. Micrococcus are obligate aerobes that produce Asama from glucose alone. Although Micrococcus live in the media contained peptone peptone but does not affect the metabolic activity of Micrococcus.In contrast, Micrococcus reacts with glucose and glucose fermentation occurs which produces lactic acid. Methyl red which previously have been incorporated into the medium becomes red due to reaction with acid. Here's a picture of red methyl red because it reacts with lactic acid:
B. Chemical Reactions Occurring
Acid fermentation of glucose produces lactic acid. Lactic acid is reacted with methyl red.
CHAPTER III: Discussion
A. The cause of occurrence of reaction
Micrococcus bacteria are bacteria that live on a medium containing peptone and glucose. However, Micrococcus only reacts with glucose and produce lactic acid which is acidic. Lactic acid is reacted with methyl red and produced a red coloration on the media.
B. Discussion of causes of occurrence of reaction Inappropriate
In this reaction no matter which causes a false-negative results or false positives.
Methyl Red (Methyl Red) is an organic compound that has the chemical formula C15H15N3O2, these compounds are widely used for acid-base indicator. This indicator is red at pH 4.4 and yellow at pH above 6.2. transition produces the color orange. Methyl Red is made with diazotisasi of antranilat acid followed by reaction with dimethylaniline. Here's a picture of making the reaction of methyl red.
Source: en.wikipedia.org / wiki / Methyl_RedB. Structure of Chemical Compounds
The chemical structure of methyl redSources: http://belajarkimia.com/kamuskimia/wp-content/uploads/2010/07/metilmerah.png
C. Chemical properties of Methyl Red
A. Tangible dark red crystalline powder.2. Stable. Incompatible with strong oxidizing agents.
D. Physical properties of compoundsMolecular formula: C15H15N3O2Molar mass: 269.3 g / molDensity: 0.791 gram/cm3Melting point: 179-182 ⁰ CDensity: 0839 g / mL at 25 ⁰ C
Chapter II: Chemical Changes in the Examination of Methyl Red Using Micrococcus2. Chemical changes in the testIn this test, the bacteria Micrococcus and media used methyl red. The goal is to know the reaction that occurs between the bacteria Micrococcus methyl red in the media. Micrococcus bacteria are gram-positive bacteria are not motile catalase positive, negative oxidation, methyl red positive, positive optimum growth temperature at 30 ⁰ -37 ⁰ C and grows well in 1-7% NaCl. Cells form a ball, diameter 0.5 to 2.0 μm, in the form of a pair, four pairs, or groups are not fixed, do not form chains. Micrococcus will usually grow on simple media. If that were cultured in agar-agar or nutrient rich, such as blood for blood. The following composition of Micrococcus isolation media:Composition per liter:
In order for 15 gramsPeptone 5 g3 grams of yeast extract1.5 grams of beef extractGlucose 1 gram
Media images of isolation for Micrococcus
A. Characteristics of Chemical Changes
Micrococcus has the aerobic capacity to produce acid from glucose glycerol, aesculin hydrolysis, arginine dihydrolase. Micrococcus are obligate aerobes that produce Asama from glucose alone. Although Micrococcus live in the media contained peptone peptone but does not affect the metabolic activity of Micrococcus.In contrast, Micrococcus reacts with glucose and glucose fermentation occurs which produces lactic acid. Methyl red which previously have been incorporated into the medium becomes red due to reaction with acid. Here's a picture of red methyl red because it reacts with lactic acid:
B. Chemical Reactions Occurring
Acid fermentation of glucose produces lactic acid. Lactic acid is reacted with methyl red.
CHAPTER III: Discussion
A. The cause of occurrence of reaction
Micrococcus bacteria are bacteria that live on a medium containing peptone and glucose. However, Micrococcus only reacts with glucose and produce lactic acid which is acidic. Lactic acid is reacted with methyl red and produced a red coloration on the media.
B. Discussion of causes of occurrence of reaction Inappropriate
In this reaction no matter which causes a false-negative results or false positives.
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